Gene Details:
- Gene ID:
- Gene Symbol: CpTGG1
- Gene Name: beta-thioglucoside glucohydrolase1
- Genome:
- Species: Carica papaya
Functional Descriptions:
- Characterization of a Novel β-thioglucosidase CpTGG1 in Carica papaya and its Substrate-dependent and Ascorbic Acid-independent O-β-glucosidase Activity.
- The recombinant CpTGG1 expressed in Pichia pastoris catalyzed the hydrolysis of both sinigrin and glucotropaeolin (the only thioglucoside present in papaya), showing that CpTGG1 was indeed a functional myrosinase gene.
- Sequence alignment analysis indicated that CpTGG1 contained all the motifs conserved in functional myrosinases from crucifers, except for two aglycon-binding motifs, suggesting substrate priority variation of the non-cruciferous myrosinases.
- CpTGG1 displayed substrate-dependent and ascorbic acid-independent O-β-glucosidase activity towards 2-nitrophenyl-β-D-glucopyranoside and 4-nitrophenyl-β-D-glucopyranoside, but was inactive towards glucovanillin and n-octyl-β-D-glucopyranoside.
Function-related keywords:
Literature:
- Characterization of a novel β-thioglucosidase CpTGG1 in Carica papaya and its substrate-dependent and ascorbic acid-independent O-β-glucosidase activity. DOI: 10.1111/j.1744-7909.2010.00988.x ; PMID: 20883440
Related News:
Gene Resources:
Orthologs:
Sequences:
- CDS Sequence:
- Protein Sequence: