Gene Details:

  • Gene ID: Seita.9G180000
  • Gene Symbol: SiGFT2
  • Gene Name: glutamate formiminotransferase 2
  • Genome:
  • Species: Setaria italica

Functional Descriptions:

  • Nullification of GFTs fortifies bioactive folates in foxtail millet.
  • The two other knockout plants carried a 1-bp insertion in SiGFT1 and a 2,946-bp deletion between the two sgRNAs that removed part of SiGFT2 (#11) and a 1-bp insertion in both SiGFT1 and SiGFT2 (#13); both combinations were predicted to successfully inactivate SiGFT1 and SiGFT2 simultaneously.
  • Additionally, the content of 5-formyltetrahydrofolate (5-formyl-THF) decreased slightly in sigft1 knockout plants, but rose by 67.6%–84.9% in the double knockout lines, suggesting that SiGFT1 and SiGFT2 have different roles in 5-formyl-THF metabolism.
  • SiGFT2 may have dual catalytic activity, like formiminotransferase-cyclodeaminase (FT-CD) in mammals.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Orthologs:

Sequences:

  • CDS Sequence:
  • Protein Sequence: