Gene Details:

• Gene ID: AT3G13170
• Gene Symbol: ATSPO11-1, SPO11-1
• Gene Name: sporulation 11-1
• Description: Spo11/DNA topoisomerase VI, subunit A protein;(source:Araport11)
• TAIR Accession:
• Genome: Araport11_genome_release
• Species: Arabidopsis thaliana

Transcripts:

• AT3G13170.1     AT3G13170.3     AT3G13170.2

Plant Ontology Annotations:

• PO:0000037  — shoot axis apex — ápice del epiblasto (epiblastema) (Spanish, exact), シュート頂、茎頂 (Japanese, exact)
• PO:0009009  — plant embryo — embrión (Spanish, exact), 植物胚 (Japanese, exact), germ (related), embryo (broad)
• PO:0009046  — flower — flor (Spanish, exact), 花 (Japanese, exact), floret (related), Asteraceae floret (narrow), basal flower (narrow), double flower (narrow), hermaphrodite flower (narrow), monoclinous flower (narrow), perfect flower (narrow)
• PO:0009052  — inflorescence flower pedicel — 小花柄 (Japanese, related), pedicelo (Spanish, broad)

Germplasm Phenotype:

• atcom1-1 atspo11-1-1  — DNA fragmentation was undetectable in most cells.
• atspo11-1-1  — 11% of mutant pollen grains germinated compared to 97% for wild type.
• atspo11-1-1  — Mutant produced a large proportion of dead pollen grains compared to wild type.
• atspo11-1-1  — Only 3% of the ovules in the mutant showed differentiated female gametophytes. 74% of the fully developed ovules had no embryo sac.
• atspo11-1-1  — Overall morphology similar to that of wild type exception made of the seed production.
• atspo11-1-1  — The mutant exhibits a substantial reduction in recombination such that the chiasma frequency at metaphase I was &
• atspo11-1-2  — 11% of mutant pollen grains germinated compared to 97% for wild type.
• atspo11-1-2  — Mutant produced a large proportion of dead pollen grains compared to wild type.
• atspo11-1-2  — Only 3% of the ovules in the mutant showed differentiated female gametophytes. 74% of the fully developed ovules had no embryo sac.
• atspo11-1-2  — Overall morphology similar to that of wild type exception made of the seed production.
• atspo11-1-3  — A 2-h pulse of cisplatin at increasing concentrations was found to restore bivalent formation in Atspo11-1-3 meiocytes.
• atspo11-1-3  — The reduction in seed-set in the Atspo11-1-3 allele is more severe than in Atspo11-1-1.
• atspo11-1-3  — Total lack of chiasmata.
• spo11-1-3 spo-2-3  — The first meiotic division of pollen mother cells because the homologs neither pair nor form SCs due to the lack of initial DSB formation

Function-related keywords:

• shoot axis apex ,  plant embryo ,  flower ,  inflorescence flower pedicel

Literature:

• ASY1 mediates AtDMC1-dependent interhomolog recombination during meiosis in Arabidopsis.   DOI:  10.1101/gad.439007 ;  PMID:  17785529
• The catalytically active tyrosine residues of both SPO11-1 and SPO11-2 are required for meiotic double-strand break induction in Arabidopsis.   DOI:  10.1105/tpc.107.054817 ;  PMID:  17965269
• A high throughput genetic screen identifies new early meiotic recombination functions in Arabidopsis thaliana.   DOI:  10.1371/journal.pgen.1000654 ;  PMID:  19763177
• A DNA-binding surface of SPO11-1, an Arabidopsis SPO11 orthologue required for normal meiosis.   DOI:  10.1111/j.1742-4658.2010.07651.x ;  PMID:  20423461
• The cyclin-A CYCA1;2/TAM is required for the meiosis I to meiosis II transition and cooperates with OSD1 for the prophase to first meiotic division transition.   DOI:  10.1371/journal.pgen.1000989 ;  PMID:  20585549
• A DNA topoisomerase VI-like complex initiates meiotic recombination.   DOI:  10.1126/science.aad5196 ;  PMID:  26917763
• MTOPVIB interacts with AtPRD1 and plays important roles in formation of meiotic DNA double-strand breaks in Arabidopsis.   DOI:  10.1038/s41598-017-10270-9 ;  PMID:  28855712
• FIGL1 and its novel partner FLIP form a conserved complex that regulates homologous recombination.   DOI:  10.1371/journal.pgen.1007317 ;  PMID:  29608566
• Meiocyte-Specific and AtSPO11-1-Dependent Small RNAs and Their Association with Meiotic Gene Expression and Recombination.   DOI:  10.1105/tpc.18.00511 ;  PMID:  30674694
• Both male and female gametogenesis require a fully functional protein S-acyl transferase 21 in Arabidopsis thaliana.   DOI:  10.1111/tpj.14475 ;  PMID:  31369173
• ATM controls meiotic DNA double-strand break formation and recombination and affects synaptonemal complex organization in plants.   DOI:  10.1093/plcell/koab045 ;  PMID:  33659989
• Heat stress interferes with formation of double-strand breaks and homolog synapsis.   DOI:  10.1093/plphys/kiab012 ;  PMID:  33793950
• Defects in meiotic chromosome segregation lead to unreduced male gametes in Arabidopsis SMC5/6 complex mutants.   DOI:  10.1093/plcell/koab178 ;  PMID:  34240187
• Heterologous Complementation of SPO11-1 and -2 Depends on the Splicing Pattern.   DOI:  10.3390/ijms22179346 ;  PMID:  34502253
• Coexpression of MEIOTIC-TOPOISOMERASE VIB-dCas9 with guide RNAs specific to a recombination hotspot is insufficient to increase crossover frequency in Arabidopsis.   DOI:  10.1093/g3journal/jkac105 ;  PMID:  35485960
• Differentiated function and localisation of SPO11-1 and PRD3 on the chromosome axis during meiotic DSB formation in Arabidopsis thaliana.   DOI:  10.1371/journal.pgen.1010298 ;  PMID:  35857772
• Tracing the evolution of the plant meiotic molecular machinery.   DOI:  10.1007/s00497-022-00456-1 ;  PMID:  36646915
• Arabidopsis SPO11-2 functions with SPO11-1 in meiotic recombination.   DOI:  10.1111/j.1365-313X.2006.02867.x ;  PMID:  17018031
• ASY1 mediates AtDMC1-dependent interhomolog recombination during meiosis in Arabidopsis.   DOI:  10.1101/gad.439007 ;  PMID:  17785529
• The catalytically active tyrosine residues of both SPO11-1 and SPO11-2 are required for meiotic double-strand break induction in Arabidopsis.   DOI:  10.1105/tpc.107.054817 ;  PMID:  17965269
• A DNA topoisomerase VI-like complex initiates meiotic recombination.   DOI:  10.1126/science.aad5196 ;  PMID:  26917763

Sequence: