Gene Details:
- Gene ID: AT4G26750
- Gene Symbol: EXT-like, LIP5
- Gene Name: extensin-like, homolog of mammalian LYST-INTERACTING PROTEIN5
- Description: hydroxyproline-rich glycoprotein family protein;(source:Araport11)
- TAIR Accession: locus:2116437
- Genome: Araport11_genome_release
- Species: Arabidopsis thaliana
Transcripts:
Plant Ontology Annotations:
- PO:0000037 — shoot axis apex — ápice del epiblasto (epiblastema) (Spanish, exact), シュート頂、茎頂 (Japanese, exact)
- PO:0008019 — leaf lamina base — base de la lámina de la hoja (Spanish, exact), 葉身基部 (Japanese, exact)
- PO:0009006 — shoot system — sistema de epiblasto (epiblastema) (Spanish, exact), シュート系、苗条系 (Japanese, exact), Poaceae crown (related), shoot (related), thalli (related), thallus (related), tree crown (narrow)
- PO:0009009 — plant embryo — embrión (Spanish, exact), 植物胚 (Japanese, exact), germ (related), embryo (broad)
- PO:0009025 — vascular leaf — foliage leaf (exact), hoja vascular (Spanish, exact), leaf, vascular (exact), vascular leaves (exact, plural), 維管束のある葉, または維管束植物の葉 (Japanese, exact), crozier (related), macrophyll (related), megaphyll (related), ascidia (narrow), ascidium (narrow), fiddlehead (narrow), frond (narrow), needle-like leaf (narrow), pitcher (narrow), pitcher blade (narrow), pitcher-blade (narrow), scale-like leaf (narrow), sterile frond (narrow), trophophyll (narrow)
- PO:0009029 — stamen — estambre (Spanish, exact), 雄蕊 (Japanese, exact), Poaceae stamen (narrow), Zea stamen (narrow)
- PO:0009031 — sepal — sépalo (Spanish, exact), がく片 (Japanese, exact)
- PO:0009046 — flower — flor (Spanish, exact), 花 (Japanese, exact), floret (related), Asteraceae floret (narrow), basal flower (narrow), double flower (narrow), hermaphrodite flower (narrow), monoclinous flower (narrow), perfect flower (narrow)
- PO:0009052 — inflorescence flower pedicel — 小花柄 (Japanese, related), pedicelo (Spanish, broad)
- PO:0020030 — cotyledon — cotiledón (Spanish, exact), seed leaf (exact), 子葉 (Japanese, exact)
- PO:0020038 — petiole — pecíolo (Spanish, exact), 葉柄 (Japanese, exact)
- PO:0020100 — hypocotyl — hipocótile (Spanish, exact), 胚軸 (Japanese, exact)
- PO:0020137 — leaf apex — ápice de la hoja (Spanish, exact), 葉先 (Japanese, exact), leaf lamina apex (narrow), phyllid apex (narrow)
- PO:0025022 — collective leaf structure — estructura colectiva de hoja (Spanish, exact), leaf series (exact), 葉が集まった構造 (Japanese, exact), leaf whorl (narrow), rosette (narrow), cycle (broad), verticil (broad)
- PO:0025281 — pollen — polen (Spanish, exact), pollen grain (exact), 花粉 (Japanese, exact)
Gene Ontology:
- GO:0005515 — enables — protein binding
- GO:1900426 — acts upstream of or within — positive regulation of defense response to bacterium
- GO:0009555 — acts upstream of or within — pollen development
- GO:0005771 — located in — multivesicular body
- GO:0042803 — enables — protein homodimerization activity
- GO:0080001 — acts upstream of or within — mucilage extrusion from seed coat
- GO:0005634 — located in — nucleus
- GO:0016192 — acts upstream of or within — vesicle-mediated transport
- GO:0098542 — involved in — defense response to other organism
- GO:0005768 — colocalizes with — endosome
- GO:1903335 — acts upstream of or within — regulation of vacuolar transport
- GO:0032511 — involved in — late endosome to vacuole transport via multivesicular body sorting pathway
- GO:0016197 — acts upstream of or within — endosomal transport
- GO:0005737 — located in — cytoplasm
- GO:0005771 — is active in — multivesicular body
Germplasm Phenotype:
- spi-4/lip5-1 — Significant reduction in seed coat mucilage. On a subcellular level there are defects in vacuolar trafficking.
Function-related keywords:
- shoot axis apex , leaf lamina base , shoot system , plant embryo , vascular leaf , stamen , sepal , flower , inflorescence flower pedicel , cotyledon , petiole , hypocotyl , leaf apex , collective leaf structure , pollen
Literature:
- DNA array analyses of Arabidopsis thaliana lacking a vacuolar Na+/H+ antiporter: impact of AtNHX1 on gene expression. DOI: 10.1111/j.1365-313X.2004.02253.x ; PMID: 15546358
- Exploring the ESCRTing machinery in eukaryotes. DOI: 10.1016/j.tplants.2006.01.008 ; PMID: 16488176
- Delayed embryo development in the ARABIDOPSIS TREHALOSE-6-PHOSPHATE SYNTHASE 1 mutant is associated with altered cell wall structure, decreased cell division and starch accumulation. DOI: 10.1111/j.1365-313X.2006.02662.x ; PMID: 16553896
- The Arabidopsis AAA ATPase SKD1 is involved in multivesicular endosome function and interacts with its positive regulator LYST-INTERACTING PROTEIN5. DOI: 10.1105/tpc.106.049346 ; PMID: 17468262
- Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection. DOI: 10.1104/pp.108.121038 ; PMID: 18650403
- Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis. DOI: 10.1104/pp.108.126375 ; PMID: 18775970
- The ESCRT-related CHMP1A and B proteins mediate multivesicular body sorting of auxin carriers in Arabidopsis and are required for plant development. DOI: 10.1105/tpc.108.064865 ; PMID: 19304934
- The AAA-type ATPase AtSKD1 contributes to vacuolar maintenance of Arabidopsis thaliana. DOI: 10.1111/j.1365-313X.2010.04310.x ; PMID: 20663085
- O-glycosylated cell wall proteins are essential in root hair growth. DOI: 10.1126/science.1206657 ; PMID: 21680836
- A novel endosomal sorting complex required for transport (ESCRT) component in Arabidopsis thaliana controls cell expansion and development. DOI: 10.1074/jbc.M113.529685 ; PMID: 24385429
- Arabidopsis LIP5, a positive regulator of multivesicular body biogenesis, is a critical target of pathogen-responsive MAPK cascade in plant basal defense. DOI: 10.1371/journal.ppat.1004243 ; PMID: 25010425
- A Critical Role of Lyst-Interacting Protein5, a Positive Regulator of Multivesicular Body Biogenesis, in Plant Responses to Heat and Salt Stresses. DOI: 10.1104/pp.15.00518 ; PMID: 26229051
- Role of SKD1 Regulators LIP5 and IST1-LIKE1 in Endosomal Sorting and Plant Development. DOI: 10.1104/pp.16.00240 ; PMID: 26983994
- Physical, Functional and Genetic Interactions between the BEACH Domain Protein SPIRRIG and LIP5 and SKD1 and Its Role in Endosomal Trafficking to the Vacuole in Arabidopsis. DOI: 10.3389/fpls.2017.01969 ; PMID: 29209342
- Arabidopsis Endoplasmic Reticulum-Localized UBAC2 Proteins Interact with PAMP-INDUCED COILED-COIL to Regulate Pathogen-Induced Callose Deposition and Plant Immunity. DOI: 10.1105/tpc.18.00334 ; PMID: 30606781
- A plant-unique ESCRT component, FYVE4, regulates multivesicular endosome biogenesis and plant growth. DOI: 10.1111/nph.17358 ; PMID: 33772801
- ESCRT components ISTL1 andLIP5 are required for tapetal function and pollen viability. DOI: 10.1093/plcell/koab132 ; PMID: 34125207
- Exploring the ESCRTing machinery in eukaryotes. DOI: 10.1016/j.tplants.2006.01.008 ; PMID: 16488176
Sequence:
cDNA Sequence
- >AT4G26750.1 GGGATTTTAGAGGTCCTTTCTGCGACGTCGTTTCTAGTTTGCAATCAGAAGCGCGTTAAATACAGGAAAATTTCTTGAGCTGTTTTGTCTTAACAAATCAATCGGAGAAAAAGCTACGGAGCTCGAGAGAAGAGAGAATCTATGTCGAACCCAAACGAACCAGCCAGGGCTCTTCTGCCTTACCTGCAACGTGCCGATGAGCTGCAGAAGCATGAACCACTTGTTGCTTATTACTGTCGGCTATATGCAATGGAACGAGGATTGAAGATTCCGCAGAGCGAGAGAACTAAGACCACCAATTCGATTCTCATGTCTCTCATTAATCAACTCGAAAAGGATAAAAAATCATTGACTCTGTCCCCAGATGACAATATGCATGTGGAGGGGTTTGCACTTAGTGTCTTTGCTAAGGCAGACAAGCAGGATCGTGCAGGAAGAGCAGACTTGGGCACTGCCAAAACTTTTTATGCTGCGAGCATCTTCTTTGAAATTCTGAGCCAGTTTGGTCCCGTACCTCCTGATATAGAGCAGAAACACAAGTATGCTGCTTGGAAGGCTGCTGACATAAGGAAAGCCATTAAAGAAGGAAGGAAGCCCACTCCAGGTGATCCTGTGGATGATGATACTGATTTATCCATCCCATCAAGCGGTCCTAGTGGCTCCTATGATCACAGTGCAAGTGATACCAACACAACAAGTCATCATAGAACAGAGCTTGATCCACCCCATGACTCGAATGATGACTCCAGCCACCATCAATTCCCTGAAGTGCCACAACACCCTCTACCTCCCAGGTTTTATGACAATCCGACCAACGATTATCCCGCAGATGTCCCACCTCCACCACCGTCTTCTTACCCTTCCAACGATCATCTTCCCCCTCCCACAGGACCATCAGACTCCCCTTACCCGCATCCTTACAGTCATCAACCATACCACCAAGACCCGCCAAAACACATGCCGCCACCGCAAAACTACTCATCTCATGAGCCTTCTCCAAATTCTCTCCCTAATTTCCAATCTTATCCTAGCTTTAGTGAGAGCAGCCTCCCATCCACTTCTCCCCACTACCCTTCTCACTACCAAAACCCAGAACCTTACTATTCTTCTCCGCACTCTGCACCTGCTCCTTCTTCCACAAGCTTCTCCTCTGCTCCTCCTCCTCCACCTTACTCATCAAACGGGCGTATCAATATTGCTCCCGTGCTAGATCCTGCACCGAGTTCAGCTCAGAAGTACCATTACGATAGCAGCTACCAGCCAGGGCCTGAGAAGGTTGCAGAGGCACTCAAGGCTGCTAGATTCGCTGTGGGAGCTTTGGCTTTTGATGAAGTCTCGACTGCTGTAGAACATCTCAAGAAGTCACTTGAGTTGCTAACAAATCCATCGGCCGGTGCCGGTCACTGAATTTTATATCTAATCTATGACACTTGGGGTTGATGTTAGTGCGTGTGTGTGTTCTCAACATTTGTGGGTTTGTTTATTAACTTTTCAGGCTCAGACTTCGTTTACAAAGAAAATTTGTGTGAATTATTCTTATTATCATAAAATTTTCCTTGCAACTTCGTGTACATTCATACATACATAGGCAATGGAGTTCCTCTTCAGTCTTCACGTAAAGAGCGAGTGTGGGACACG
CDS Sequence
Protein Sequence