Gene Details:

• Gene ID: AT5G24630
• Gene Symbol: BIN4, MID
• Gene Name: brassinosteroid-insensitive4, MIDGET
• Description: double-stranded DNA binding protein;(source:Araport11)
• TAIR Accession: locus:2153939
• Genome: Araport11_genome_release
• Species: Arabidopsis thaliana

Transcripts:

• AT5G24630.1     AT5G24630.2     AT5G24630.3     AT5G24630.4     AT5G24630.5     AT5G24630.6     AT5G24630.11     AT5G24630.12     AT5G24630.9     AT5G24630.8     AT5G24630.7     AT5G24630.10

Gene Ontology:

• GO:0010090  — acts upstream of or within — trichome morphogenesis
• GO:0048364  — acts upstream of or within — root development
• GO:0009330  — part of — DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex
• GO:0042023  — involved in — DNA endoreduplication
• GO:0030307  — acts upstream of or within — positive regulation of cell growth
• GO:0051276  — acts upstream of or within — chromosome organization
• GO:0042023  — acts upstream of or within — DNA endoreduplication
• GO:0003690  — enables — double-stranded DNA binding
• GO:0090627  — acts upstream of or within — plant epidermal cell differentiation
• GO:0048367  — acts upstream of or within — shoot system development
• GO:0005634  — is active in — nucleus
• GO:0005634  — located in — nucleus
• GO:0051276  — involved in — chromosome organization

Germplasm Phenotype:

• bin4-1  — Various organs of this brassinolide-insensitive dwarf mutant, such as leaves and roots, are only about 25% the size of wild-type organs. Their cells have smaller nuclei and reduced ploidy, based on analyses of 12-day old leaves. bin4-1 phenotypes are not altered in triptychon, rastafari, kaktus2, or glabra2 mutants. However, chromosome doubling induced by colchicine does lead to increased cell size. SPO11-3/RHL2/BIN5:GFP can still enter the nucleus in the bin4-1 mutant background. The transcript level of several DSB-inducible genes, such as PARP1 is higher in bin4-1 mutants, but these mutants do not show general defects in the maintenance of transcriptional gene silencing. Elevated levels of DSB-responsive transcripts in bin4-1 can be partially suppressed by atm or atr mutations. There is ectopic expression of the transcriptional CYCB1;1:GUS reporter in non-dividing cells in the bin4-1 mutant. However, the transcript levels of many other G2/M genes are not altered based on RT-PCR analysis.
• bin4-2  — The bin4-2 mutant has a dwarf phenotype with reduced cell size and a reduction in the maximum amount of endoreduplication observed in leaf cells. Cotyledon cells from bin4-2 mutants have higher levels of double strand breaks than comparable wild-type cotyledon cells.
• mid-1  — Small plants, reduced cell size, decreased endoreduplication, reduced number of root hairs and abnormal development of columella cells of the seed coat.
• mid-2  — Small plants, reduced cell size, decreased endoreduplication, reduced number of root hairs and abnormal development of columella cells of the seed coat.

Literature:

• MIDGET unravels functions of the Arabidopsis topoisomerase VI complex in DNA endoreduplication, chromatin condensation, and transcriptional silencing.   DOI:  10.1105/tpc.107.054361 ;  PMID:  17951446
• Systematic identification of functional plant modules through the integration of complementary data sources.   DOI:  10.1104/pp.112.196725 ;  PMID:  22589469
• MIDGET connects COP1-dependent development with endoreduplication in Arabidopsis thaliana.   DOI:  10.1111/tpj.12199 ;  PMID:  23573936
• MIDGET cooperates with COP1 and SPA1 to repress flowering in Arabidopsis thaliana.   DOI:  10.4161/psb.25600 ;  PMID:  23857347
• A crucial role for the putative Arabidopsis topoisomerase VI in plant growth and development.   DOI:  10.1073/pnas.152337599 ;  PMID:  12119417
• MIDGET unravels functions of the Arabidopsis topoisomerase VI complex in DNA endoreduplication, chromatin condensation, and transcriptional silencing.   DOI:  10.1105/tpc.107.054361 ;  PMID:  17951446

Sequence: