Gene Details:

• Gene ID:
• Gene Symbol: MdOSC4
• Gene Name: oxidosqualene cyclase4
• Genome: Golden ASM211411v1
• Species: Malus domestica

Functional Descriptions:

• We used a homology-based approach to identify and functionally characterize two new oxidosqualene cyclases (MdOSC4 and MdOSC5) and one cytochrome P450 (CYP716A175).
• MdOSC4 encodes a multifunctional oxidosqualene cyclase producing an oleanane-type triterpene, putatively identified as germanicol, as well as β-amyrin and lupeol, in the proportion 82 : 14 : 4. MdOSC5 cyclizes 2,3-oxidosqualene into lupeol and β-amyrin at a ratio of 95 : 5.
• The gene expression of four OSC genes (MdOSC1, MdOSC3, and MdOSC4 and MdOSC5) as well as the cytochrome P450 was also measured in a collection of 20 contrasting apple cultivars (in terms of triterpene accumulation) and revealed the key roles played by MdOSC1 and MdOSC5 in explaining the concentrations of α-amyrin and lupeol derivatives, respectively.
• Phylogenetic analysis showed that MdOSC4 and MdOSC5 lie among the β-amyrin synthases and other mixed functional triterpene synthases and away from identified lupeol synthases.
• The transient expression of MdOSC4 in N. benthamiana led to the formation of a mixture of triterpenes dominated by an oleanane-type triterpene, putatively identified as germanicol (82%).

Function-related keywords:

Literature:

• Multifunctional oxidosqualene cyclases and cytochrome P450 involved in the biosynthesis of apple fruit triterpenic acids.   DOI:  10.1111/nph.13996 ;  PMID:  27214242

Related News:

Gene Resources:

• NCBI ID: KT383435
• UniProt accessions:

Orthologs:

Sequences: