Gene Details:

Functional Descriptions:

  • Earlier, we reported the isolation of OsiIAA1 cDNA, first monocot member of Aux/IAA gene family from rice.
  • Extending this work further, we have isolated the OsiIAA1 gene from rice localized on chromosome 3.
  • The increased accumulation of OsiIAA1 transcript in auxin-treated rice coleoptiles even in the presence of a protein synthesis inhibitor, cycloheximide, suggested that OsiIAA1 is a primary auxin response gene; the expression of OsiIAA1 gene was also upregulated in the presence of cycloheximide alone.
  • The OsiIAA1 transcript levels were down-regulated in etiolated rice coleoptiles irradiated with far-red, red and blue light, suggesting the existence of a cross-talk between auxin and light signaling.
  • The antibodies raised against His6-OsiIAA1 recombinant protein could detect the OsiIAA1 protein in the plant extract only in the presence of a proteasome inhibitor, MG132, indicating that OsiIAA1 is rapidly degraded by proteasome complex.
  • Also, the proteasome inhibitor MG132 stabilized the purified His6-OsiIAA1 protein to some extent in the cell-free extracts of rice coleoptiles.
  • The OsiIAA1 protein harbors two nuclear localization signals (NLSs), one bipartite and the other resembling SV40 type NLS.
  • These studies indicate that nuclear localization of OsiIAA1 could be a prerequisite for its role in auxin signal transduction.
  • Exogenous auxin enhances the degradation of a light down-regulated and nuclear-localized OsiIAA1, an Aux/IAA protein from rice, via proteasome.
  • In this study, we newly isolated and characterized a rice gain-of-function mutant, OsIAA13, containing a single amino acid substitution in the core sequence required for the degradation of the OsIAA13 protein.
  • The OsIAA13 mutant displayed typical auxin-related phenotypes: the number of lateral roots was significantly reduced and the root gravitropic response was defective.
  • OsIAA13 mutants also exhibited altered GUS staining controlled by the DR5 promoter in lateral root initiation sites.
  • Furthermore, expression levels of several genes that might be associated with lateral root initiation were altered in OsIAA13.
  • Taken together, our results indicate that OsIAA13 is involved in auxin signaling and controls the expression of genes that are required for lateral root initiation in rice.
  • OsIAA13-mediated auxin signaling is involved in lateral root initiation in rice.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os03g53150.2 cDNA CTTCCCTCTCTCTATATATATACTCATCTCCAAGCTGTTCTTGGCTCCTCTCTCTTCTTCCAACACCTCCTAATCACCACAAAGGCTTAAAAATCTCCAGTCTCCATAGAGAGAAAGAGAGAGAAGAGAAGGCAGCTTGATTTGGGGCGAGGAGACGCGATAGCCGCGATAGGCTAAGCAGCTGGCAGCGGCGGCTGCTGCGATGGCCGGCGCCGACGTGGACGTCGGGACGGAGCTCCGGCTGGGGCTGCCCGGAGGTGGCGGCGGCGCCGCCGAGGCGGCGGCCAAGGCCGCGAAGAGGGGCTTCGAGGAGACCATTGACCTGAAGCTGAAGCTGCCCACCGCCGGCATGGAGGAGGCCGCCGCCGGCAAGGCGGAGGCGCCGGCCGCCGAGAAGGCCAAGAGGCCGGCGGAGGCCGCCGCCGCCGACGCCGAGAAGCCACCTGCTCCCAAGGCGCAGGCAGTGGGTTGGCCACCAGTTCGGTCGTTCCGCAGGAACATCATGACCGTCCAGTCAGTGAAGAGCAAGAAGGAAGAGGAAGCTGACAAGCAGCAGCAGCAGCCCGCTGCCAATGCCAGCGGCAGCAACAGCTCTGCCTTCGTGAAGGTCAGCATGGACGGGGCACCCTACCTGCGCAAGGTGGACCTGAAGATGTACAACAGCTACAAGGACCTCTCCCTTGCTCTGCAGAAGATGTTCGGCACCTTCACCGCAACTGGGAACAATATGAATGAGGTGAATGGCTCTGATGCTGTTACAACTTATGAAGACAAGGATGGTGACTGGATGCTTGTTGGAGATGTCCCGTGGCAGTATGTTACTACTTCATTCTTGTGCCCCCTTAACTGTTTTCCTTATTTTCCACCTCATGATTAGAATGCGGTTTAGCGATACCACGTTCAAGTTTCTCGCATTAGCTGATTGGTTACTCTGCAGGATGTTTGTCGAGTCATGCAAACGCTTGAGGATCATGAAGGGTTCCGAAGCCATTGGTCTTGGTCAGTATCTTCAGCTAAAATTTGCTTTCGTGTCAAGATTTATATCTTAATTCACACCCCTTGTGTGTGTGGTTTTTCTTCCAGCACCAAGAGCAAAGGACAAGTACAAGAACAAGAGCTGAAAATGTTGCAGTATTAAGATGCTGAGCATGGTGCCATAAGAATGGTGTTCTTGCAGTGTGTTATGGTTTCCTTAGAAATATAAGGTTTTACTTGTTTAGCTGGATTAAAAGAAGGAAGAGTTGTCATGTTTAATTTGTTTATGGACCAGAAGCAATAATCTATTTGTGTGTAGCTGGATTGGTGTTATGTGATGTCCCCTACCGTGTGTCCCAAGATCTACTTGATGATTTTATTCCAATGTTGTTAATTTAAGTTTGTGTGATTGTGAAGTAAAGGGTATCAATGAGACTATTATTATTATGGTATCTGGTAAGTTAACATGTTTGTTAATCATCAGCACAATATTTTTGTAA
  • >LOC_Os03g53150.1 cDNA CTTCCCTCTCTCTATATATATACTCATCTCCAAGCTGTTCTTGGCTCCTCTCTCTTCTTCCAACACCTCCTAATCACCACAAAGGCTTAAAAATCTCCAGTCTCCATAGAGAGAAAGAGAGAGAAGAGAAGGCAGCTTGATTTGGGGCGAGGAGACGCGATAGCCGCGATAGGCTAAGCAGCTGGCAGCGGCGGCTGCTGCGATGGCCGGCGCCGACGTGGACGTCGGGACGGAGCTCCGGCTGGGGCTGCCCGGAGGTGGCGGCGGCGCCGCCGAGGCGGCGGCCAAGGCCGCGAAGAGGGGCTTCGAGGAGACCATTGACCTGAAGCTGAAGCTGCCCACCGCCGGCATGGAGGAGGCCGCCGCCGGCAAGGCGGAGGCGCCGGCCGCCGAGAAGGCCAAGAGGCCGGCGGAGGCCGCCGCCGCCGACGCCGAGAAGCCACCTGCTCCCAAGGCGCAGGCAGTGGGTTGGCCACCAGTTCGGTCGTTCCGCAGGAACATCATGACCGTCCAGTCAGTGAAGAGCAAGAAGGAAGAGGAAGCTGACAAGCAGCAGCAGCAGCCCGCTGCCAATGCCAGCGGCAGCAACAGCTCTGCCTTCGTGAAGGTCAGCATGGACGGGGCACCCTACCTGCGCAAGGTGGACCTGAAGATGTACAACAGCTACAAGGACCTCTCCCTTGCTCTGCAGAAGATGTTCGGCACCTTCACCGCAACTGGGAACAATATGAATGAGGTGAATGGCTCTGATGCTGTTACAACTTATGAAGACAAGGATGGTGACTGGATGCTTGTTGGAGATGTCCCGTGGCAGTATGTTACTACTTCATTCTTGTGCCCCCTTAACTGTTTTCCTTATTTTCCACCTCATGATTAGAATGCGGTTTAGCGATACCACGTTCAAGTTTCTCGCATTAGCTGATTGGTTACTCTGCAGGATGTTTGTCGAGTCATGCAAACGCTTGAGGATCATGAAGGGTTCCGAAGCCATTGGTCTTGGTCAGTATCTTCAGCTAAAATTTGCTTTCGTGTCAAGATTTATATCTTAATTCACACCCCTTGTGTGTGTGGTTTTTCTTCCAGCACCAAGAGCAAAGGACAAGTACAAGAACAAGAGCTGAAAATGTTGCAGTATTAAGATGCTGAGCATGGTGCCATAAGAATGGTGTTCTTGCAGTGTGTTATGGTTTCCTTAGAAATATAAGGTTTTACTTGTTTAGCTGGATTAAAAGAAGGAAGAGTTGTCATGTTTAATTTGTTTATGGACCAGAAGCAATAATCTATTTGTGTGTAGCTGGATTGGTGTTATGTGATGTCCCCTACCGTGTGTCCCAAGATCTACTTGATGATTTTATTCCAATGTTGTTAATTTAAGTTTGTGTGATTGTGAAGTAAAGGGTATCAATGAGACTATTATTATTATGGTATCTGGTAAGTTAACATGTTTGTTAATCATCAGCACAATATTTTTGTAA
CDS Sequence
  • >LOC_Os03g53150.2 CDS ATGGCCGGCGCCGACGTGGACGTCGGGACGGAGCTCCGGCTGGGGCTGCCCGGAGGTGGCGGCGGCGCCGCCGAGGCGGCGGCCAAGGCCGCGAAGAGGGGCTTCGAGGAGACCATTGACCTGAAGCTGAAGCTGCCCACCGCCGGCATGGAGGAGGCCGCCGCCGGCAAGGCGGAGGCGCCGGCCGCCGAGAAGGCCAAGAGGCCGGCGGAGGCCGCCGCCGCCGACGCCGAGAAGCCACCTGCTCCCAAGGCGCAGGCAGTGGGTTGGCCACCAGTTCGGTCGTTCCGCAGGAACATCATGACCGTCCAGTCAGTGAAGAGCAAGAAGGAAGAGGAAGCTGACAAGCAGCAGCAGCAGCCCGCTGCCAATGCCAGCGGCAGCAACAGCTCTGCCTTCGTGAAGGTCAGCATGGACGGGGCACCCTACCTGCGCAAGGTGGACCTGAAGATGTACAACAGCTACAAGGACCTCTCCCTTGCTCTGCAGAAGATGTTCGGCACCTTCACCGCAACTGGGAACAATATGAATGAGGTGAATGGCTCTGATGCTGTTACAACTTATGAAGACAAGGATGGTGACTGGATGCTTGTTGGAGATGTCCCGTGGCAGTATGTTACTACTTCATTCTTGTGCCCCCTTAACTGTTTTCCTTATTTTCCACCTCATGATTAG
  • >LOC_Os03g53150.1 CDS ATGGCCGGCGCCGACGTGGACGTCGGGACGGAGCTCCGGCTGGGGCTGCCCGGAGGTGGCGGCGGCGCCGCCGAGGCGGCGGCCAAGGCCGCGAAGAGGGGCTTCGAGGAGACCATTGACCTGAAGCTGAAGCTGCCCACCGCCGGCATGGAGGAGGCCGCCGCCGGCAAGGCGGAGGCGCCGGCCGCCGAGAAGGCCAAGAGGCCGGCGGAGGCCGCCGCCGCCGACGCCGAGAAGCCACCTGCTCCCAAGGCGCAGGCAGTGGGTTGGCCACCAGTTCGGTCGTTCCGCAGGAACATCATGACCGTCCAGTCAGTGAAGAGCAAGAAGGAAGAGGAAGCTGACAAGCAGCAGCAGCAGCCCGCTGCCAATGCCAGCGGCAGCAACAGCTCTGCCTTCGTGAAGGTCAGCATGGACGGGGCACCCTACCTGCGCAAGGTGGACCTGAAGATGTACAACAGCTACAAGGACCTCTCCCTTGCTCTGCAGAAGATGTTCGGCACCTTCACCGCAACTGGGAACAATATGAATGAGGTGAATGGCTCTGATGCTGTTACAACTTATGAAGACAAGGATGGTGACTGGATGCTTGTTGGAGATGTCCCGTGGCAGTATGTTACTACTTCATTCTTGTGCCCCCTTAACTGTTTTCCTTATTTTCCACCTCATGATTAG
Protein Sequence
  • >LOC_Os03g53150.2 protein MAGADVDVGTELRLGLPGGGGGAAEAAAKAAKRGFEETIDLKLKLPTAGMEEAAAGKAEAPAAEKAKRPAEAAAADAEKPPAPKAQAVGWPPVRSFRRNIMTVQSVKSKKEEEADKQQQQPAANASGSNSSAFVKVSMDGAPYLRKVDLKMYNSYKDLSLALQKMFGTFTATGNNMNEVNGSDAVTTYEDKDGDWMLVGDVPWQYVTTSFLCPLNCFPYFPPHD*
  • >LOC_Os03g53150.1 protein MAGADVDVGTELRLGLPGGGGGAAEAAAKAAKRGFEETIDLKLKLPTAGMEEAAAGKAEAPAAEKAKRPAEAAAADAEKPPAPKAQAVGWPPVRSFRRNIMTVQSVKSKKEEEADKQQQQPAANASGSNSSAFVKVSMDGAPYLRKVDLKMYNSYKDLSLALQKMFGTFTATGNNMNEVNGSDAVTTYEDKDGDWMLVGDVPWQYVTTSFLCPLNCFPYFPPHD*