Gene Details:

• MSU gene ID: LOC_Os07g26630
• RAPdb gene ID: Os07g0448100
• Gene Symbol: OsPIP2 4
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• Two rice plasma membrane intrinsic proteins, OsPIP2;4 and OsPIP2;7, are involved in transport and providing tolerance to boron toxicity.
• Transcript analysis demonstrated that the expression of OsPIP2;4 and OsPIP2;7 were downregulated in shoots and strongly upregulated in rice roots by high B treatment.
• Arabidopsis lines overexpressing OsPIP2;4 and OsPIP2;7 showed significantly higher biomass production and greater root length, however there was no difference in B accumulation in long term uptake assay.
• Short-term uptake assay using tracer B ((1)(0)B) in shoots and roots demonstrated increased (1)(0)B accumulation in Arabidopsis lines expressing OsPIP2;4 and OsPIP2;7, compare to wild type control plants.
• Efflux assay of B in the roots showed that (1)(0)B was effluxed from the Arabidopsis transgenic plants overexpressing OsPIP2;4 or OsPIP2;7 during the initial 1-h of assay.
• These data indicate that OsPIP2;4 and OsPIP2;7 are involved in mediating B transport in rice and provide tolerance via efflux of excess B from roots and shoot tissues.
• Overexpression of OsPIP2;4, OsPIP2;6, and OsPIP2;7 in Arabidopsis yielded enhanced arsenite tolerance and higher biomass accumulation.
• qRT-PCR analysis of PIPs in rice root and shoot tissues revealed a significant down regulation of transcripts encoding OsPIP1;2, OsPIP1;3, OsPIP2;4, OsPIP2;6, and OsPIP2;7 in response to arsenite treatment.
• Heterologous expression of OsPIP2;4, OsPIP2;6, and OsPIP2;7 in Xenopus laevis oocytes significantly increased the uptake of arsenite.
• Expression levels of two root-specific aquaporin genes, OsPIP2;4 and OsPIP2;5, were increased significantly after 6 and 13 d of LRT exposure.

Function-related keywords:

• boron ,  root ,  shoot ,  biomass ,  arsenite

Literature:

• Two rice plasma membrane intrinsic proteins, OsPIP2;4 and OsPIP2;7, are involved in transport and providing tolerance to boron toxicity .  DOI:  10.1007/s00425-013-1969-y ;  PMID:  24142111
• The role of water channel proteins and nitric oxide signaling in rice seed germination .  DOI:  10.1038/cr.2007.34 ;  PMID:  17452999
• Influence of low air humidity and low root temperature on water uptake, growth and aquaporin expression in rice plants .  DOI:  10.1093/pcp/pcs087 ;  PMID:  22685088
• Members of rice plasma membrane intrinsic proteins subfamily are involved in arsenite permeability and tolerance in plants .  DOI:  10.1007/s11248-012-9600-8 ;  PMID:  22350764

Related News:

Gene Resources:

• NCBI ID: AK072632
• UniProt accessions:

Sequences: