Gene Details:

Functional Descriptions:

  • PHOSPHOENOLPYRUVATE CARBOXYLASE 2 (PPC2), enabling generation of plants with fruit-specific gene editing inherited through the next generation.
  • PPC2-driven fruit gene editing can be efficiently performed and coupled to development of transgene seeds, which after seedling sequencing can be determined either to harbor non-edited (functional) target gene for maintenance of fruit specificity in successive generations, in addition to edited (nonfunctional alleles) for analysis of germline mutations.
  • CRISPR-Cas9-mediated disruption of GFP driven by the PPC2 promoter showed fruit specificity in targeted disruption.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • />Solyc07g062530.3.1
    AATACATACCATGAAATGACAAAGTGACAAAGTAGAGTGTAAGAGCTTATTATATAGTGAACAATTCTCTCTTTTAGATCACAAATCCCTTGAACAAACAAAGCAAAAACCAAATCATTTTTCTAAGTTAAAAAAAAAAAATCTGATTTTTGTTTTTCTTACTGGAAAAAAGCTTCTTTTTTTTCCTTTCTTCATCATCTGGGTTCTAAAATAAATCAAGATTCAGGATTTTGATTTTGTAGTTTGAGTAAAAAGGGGGTTGATGGCTACTCGTAATTTGGAGAAATTTGCATCTATAGATGCACAGATGAGGCTTTTGGTACCTACTAAGGTGTCTGAAGATGATATGCTTGTTGAGTATGATGCTTTGCTTTTGGATCGATTTCTTGATATTCTTGAGGATTTGCATGGAGAGGGTATCAAAACAACAGTCCAAGAATGTTATGAGCTTTCTGCTGAGTATGAAAACACGCATGATAAGAAGAAGCTGGAGGAGCTTGGCAGTGTGTTGACAACTTTGGATCCAGGGGATTCAATTGTTGTTGCTAAGTCGATATCCCATATGCTTAACATGGCCAACTTGGCTGAGGAGGTTCAGATTGCCAACAGTGGGAGGGCAAAGTCGAAAAAGGGCGAACTTTCAGATGAGAGCAACTTGATGACTGAATCCGACATTGAAGAAACACTCAAGAGACTTGTAGTGAACTTGAACAAGTCTCCTCGAGAAGTTTTTAATGCTCTGAAGAATCAGACTGTGGATTTGGTCTTTACTGCTCATCCGACTCAAGCTATCCGAAGATCTTTGCTTCAGAAGCATGCAAGGATCCGCAATTGTTTGTCTCAGTTGTATGCTAAAGACATTACACCCGACGATAAAGAGGAGCTGGATGAAGCTTTAGAGAGGGAGATTCAAGCTGCTTTCCGCACTGATGAAATCAGAAGGACTCCTCCAACACCACAAGATGAAATGAGAGCTGGAATGAGTTACTTCCATGAAACAATTTGGAACGGTGTTCCGAAGTTTCTACGACGTGTAGACACAGCTCTTAAAAACATAGGGATTGATGAACGAGTTCCTTATAATGCTCCTCTCATTCAGTTCTCGTCTTGGATGGGTGGCGACCGTGATGGTAATCCAAGAGTAACTCCTGAGGTCACTAGAGATGTTTGCTTATTGGCCCGAATGATGGCAGCGAATTTGTACTACTCTCAGATAGATGATCTAATGTTTGAGTTATCTATGTGGCGTTGCAATGACGAGCTTCGTGATCGGGCAGATGAACTACACCGTTCATTAAAGAGAGACGAAAAACACTATATAGAATTCTGGAAACAAGTTCCTCCAAGTGAACCATATCGTGTAATTATTGGTGATGTGAGAGATAAGCTATATCATACACGTGAGCGTACTCGCCAACTGTTATCCAATGGGTTCTCTGAAATTCCTGAGGAAGCAACATATACTAACATCAAGCAGTTCTTGGAACCTCTTGAGCTCTGCTACAGATCTCTTTGTGCTTGTGGGGATCGACCAATTGCTGACGGAAGCCTTCTGGATTTTCTACGACAAGTTTCTACCTTTGGACTCTCACTTGTGAGACTTGACATAAGACAAGAGTCGGATCGGCACACTGATGTCCTTGATGCCATTACCCAGCACTTGGAAATTGGTTCATATCGAGAGTGGTCTGAGGAACGTAAACAAGAGTGGCTTCTCTCTGAACTCAGCGGCAAGCGACCTCTATTTGGACCCGACCTACCAAAAACTGAAGAAATTGATGATGTTTTGAATACATTCCATGTCATATCAGAACTTCCATCAGACTGCTTTGGGGCATACATCATCTCAATGGCCACTGCACCATCTGATGTGCTTGCTGTTGAGCTTCTACAGCGTGAATGCCACGTGAAGCAACCTTTACGGGTAGTTCCACTTTTTGAGAAATTGGATGATCTGGATGCTGCTCCTGCTGCTATTGCACGTCTTTTTTCAATTGAGTGGTACAAAAACCGCATTAATGGGAAGCAAGAAGTCATGATTGGCTACTCAGACTCTGGAAAGGATGCTGGTCGGCTGTCGGCAGCATGGCAGCTATATAAGGCTCAAGAGGAGCTTATAAAAGTTGCCAAACAATATGACGTGAAGCTAATTATGTTCCATGGCAGAGGTGGTACAGTTGGAAGAGGAGGTGGTCCTAGCCATCTTGCTATATTATCTCAGCCACCTGATACGATTCAGGGATCTCTTCGCGTAACAGTTCAAGGTGAGGTTATTGAGCAATCATTTGGGGAGGAACACTTGTGTTTTAGGACACTCCAACGTTTCACTTCTGCCACCCTAGAACATGGAATGTATCCACCTTTCCCCCCAAAGCCAGAATGGCGTGCACTTCTGGATGAAATTTCTGTTGTTGCTACAGAGAAGTATAGGTCAATAGTATTCAAGGAACCCCGATTTGTCGAGTACTTCCGCCTGGCAACACCCGAGCTGGAGTATGGTCGAATGAACATTGGTAGCCGTCCATCAAAGCGTAAACGAAATGGAGGCATAGAAACACTTAGAGCCATTCCATGGATATTTGCATGGACCCAGACTAGGTTTCATCTGCCGGTCTGGCTTGGCTTTGGGGCAGCATTTAAGTATGCACTTAACAAGGATAAAAAAAACCTCAATATGCTGCGGGACATGCACAAAAAATGGCCATTTTTTAGAGTTACTGCTGATTTGATTGAGATGGTGTTTGCTAAGGGAGACCCCGGCATTGCTGCTTTATACGACAAGCTTCTGGTTTCTGAAGATTTGTGGTCCTTCGGTGAGCTTTTGAGGTCTGACTATGAGGAGACAAAGAGCCTCCTGCTTAAGGTTGCTGGACACAAGGAACTTCTGGAGAACGATCCCTCCTTAAAACAACGACTCAGGCTGCGTGATTCCTATATCACTACTTTAAATGTCTGCCAAGCTTACACACTAAAGAGGATTCGTGATCCAACCTACAAAGTCACTCCAAGGCCTCACATTACCAAGGAATACATAGAATCGAAACCTGCTGCTGAACTCGTGAGCCTGAACCCACAAAGCGAATATGCTCCCGGCTTGGAAGACACACTCATCTTGACCATGAAAGGTATTGCTGCTGGAATGCAGAATACTGGTTAAATCTATTACTATGTTTTTATGTTTTAATGGTTGATAAAGTGAACATTAAGAATCATGTATCCTTGAGTTCATCTTTTTTTTTTTTTTGCAACTTCCATAAAATGGATGAATAATTATTCCCCTCTTTGTTTAGTAGCTTCTTGAATAAAATTATGATGTAAAAGAGTACTTCTCTTCATTTACCAAATGCTTTTTGCAATTAATCTCAGTTCCAATCTTTCAGTTTTATATTTAATAGTATTTGTCATTTTAAAATAAAATAAAATCATCTCACTATTATGAAGGTTCATTTCTCGAGTAATATATATGGATGTATTATTTAAATGAAAAAAATAATAAAAA
CDS Sequence
  • />Solyc07g062530.3.1
    ATGGCTACTCGTAATTTGGAGAAATTTGCATCTATAGATGCACAGATGAGGCTTTTGGTACCTACTAAGGTGTCTGAAGATGATATGCTTGTTGAGTATGATGCTTTGCTTTTGGATCGATTTCTTGATATTCTTGAGGATTTGCATGGAGAGGGTATCAAAACAACAGTCCAAGAATGTTATGAGCTTTCTGCTGAGTATGAAAACACGCATGATAAGAAGAAGCTGGAGGAGCTTGGCAGTGTGTTGACAACTTTGGATCCAGGGGATTCAATTGTTGTTGCTAAGTCGATATCCCATATGCTTAACATGGCCAACTTGGCTGAGGAGGTTCAGATTGCCAACAGTGGGAGGGCAAAGTCGAAAAAGGGCGAACTTTCAGATGAGAGCAACTTGATGACTGAATCCGACATTGAAGAAACACTCAAGAGACTTGTAGTGAACTTGAACAAGTCTCCTCGAGAAGTTTTTAATGCTCTGAAGAATCAGACTGTGGATTTGGTCTTTACTGCTCATCCGACTCAAGCTATCCGAAGATCTTTGCTTCAGAAGCATGCAAGGATCCGCAATTGTTTGTCTCAGTTGTATGCTAAAGACATTACACCCGACGATAAAGAGGAGCTGGATGAAGCTTTAGAGAGGGAGATTCAAGCTGCTTTCCGCACTGATGAAATCAGAAGGACTCCTCCAACACCACAAGATGAAATGAGAGCTGGAATGAGTTACTTCCATGAAACAATTTGGAACGGTGTTCCGAAGTTTCTACGACGTGTAGACACAGCTCTTAAAAACATAGGGATTGATGAACGAGTTCCTTATAATGCTCCTCTCATTCAGTTCTCGTCTTGGATGGGTGGCGACCGTGATGGTAATCCAAGAGTAACTCCTGAGGTCACTAGAGATGTTTGCTTATTGGCCCGAATGATGGCAGCGAATTTGTACTACTCTCAGATAGATGATCTAATGTTTGAGTTATCTATGTGGCGTTGCAATGACGAGCTTCGTGATCGGGCAGATGAACTACACCGTTCATTAAAGAGAGACGAAAAACACTATATAGAATTCTGGAAACAAGTTCCTCCAAGTGAACCATATCGTGTAATTATTGGTGATGTGAGAGATAAGCTATATCATACACGTGAGCGTACTCGCCAACTGTTATCCAATGGGTTCTCTGAAATTCCTGAGGAAGCAACATATACTAACATCAAGCAGTTCTTGGAACCTCTTGAGCTCTGCTACAGATCTCTTTGTGCTTGTGGGGATCGACCAATTGCTGACGGAAGCCTTCTGGATTTTCTACGACAAGTTTCTACCTTTGGACTCTCACTTGTGAGACTTGACATAAGACAAGAGTCGGATCGGCACACTGATGTCCTTGATGCCATTACCCAGCACTTGGAAATTGGTTCATATCGAGAGTGGTCTGAGGAACGTAAACAAGAGTGGCTTCTCTCTGAACTCAGCGGCAAGCGACCTCTATTTGGACCCGACCTACCAAAAACTGAAGAAATTGATGATGTTTTGAATACATTCCATGTCATATCAGAACTTCCATCAGACTGCTTTGGGGCATACATCATCTCAATGGCCACTGCACCATCTGATGTGCTTGCTGTTGAGCTTCTACAGCGTGAATGCCACGTGAAGCAACCTTTACGGGTAGTTCCACTTTTTGAGAAATTGGATGATCTGGATGCTGCTCCTGCTGCTATTGCACGTCTTTTTTCAATTGAGTGGTACAAAAACCGCATTAATGGGAAGCAAGAAGTCATGATTGGCTACTCAGACTCTGGAAAGGATGCTGGTCGGCTGTCGGCAGCATGGCAGCTATATAAGGCTCAAGAGGAGCTTATAAAAGTTGCCAAACAATATGACGTGAAGCTAATTATGTTCCATGGCAGAGGTGGTACAGTTGGAAGAGGAGGTGGTCCTAGCCATCTTGCTATATTATCTCAGCCACCTGATACGATTCAGGGATCTCTTCGCGTAACAGTTCAAGGTGAGGTTATTGAGCAATCATTTGGGGAGGAACACTTGTGTTTTAGGACACTCCAACGTTTCACTTCTGCCACCCTAGAACATGGAATGTATCCACCTTTCCCCCCAAAGCCAGAATGGCGTGCACTTCTGGATGAAATTTCTGTTGTTGCTACAGAGAAGTATAGGTCAATAGTATTCAAGGAACCCCGATTTGTCGAGTACTTCCGCCTGGCAACACCCGAGCTGGAGTATGGTCGAATGAACATTGGTAGCCGTCCATCAAAGCGTAAACGAAATGGAGGCATAGAAACACTTAGAGCCATTCCATGGATATTTGCATGGACCCAGACTAGGTTTCATCTGCCGGTCTGGCTTGGCTTTGGGGCAGCATTTAAGTATGCACTTAACAAGGATAAAAAAAACCTCAATATGCTGCGGGACATGCACAAAAAATGGCCATTTTTTAGAGTTACTGCTGATTTGATTGAGATGGTGTTTGCTAAGGGAGACCCCGGCATTGCTGCTTTATACGACAAGCTTCTGGTTTCTGAAGATTTGTGGTCCTTCGGTGAGCTTTTGAGGTCTGACTATGAGGAGACAAAGAGCCTCCTGCTTAAGGTTGCTGGACACAAGGAACTTCTGGAGAACGATCCCTCCTTAAAACAACGACTCAGGCTGCGTGATTCCTATATCACTACTTTAAATGTCTGCCAAGCTTACACACTAAAGAGGATTCGTGATCCAACCTACAAAGTCACTCCAAGGCCTCACATTACCAAGGAATACATAGAATCGAAACCTGCTGCTGAACTCGTGAGCCTGAACCCACAAAGCGAATATGCTCCCGGCTTGGAAGACACACTCATCTTGACCATGAAAGGTATTGCTGCTGGAATGCAGAATACTGGTTAA
Protein Sequence
  • />Solyc07g062530.3.1
    MATRNLEKFASIDAQMRLLVPTKVSEDDMLVEYDALLLDRFLDILEDLHGEGIKTTVQECYELSAEYENTHDKKKLEELGSVLTTLDPGDSIVVAKSISHMLNMANLAEEVQIANSGRAKSKKGELSDESNLMTESDIEETLKRLVVNLNKSPREVFNALKNQTVDLVFTAHPTQAIRRSLLQKHARIRNCLSQLYAKDITPDDKEELDEALEREIQAAFRTDEIRRTPPTPQDEMRAGMSYFHETIWNGVPKFLRRVDTALKNIGIDERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTRDVCLLARMMAANLYYSQIDDLMFELSMWRCNDELRDRADELHRSLKRDEKHYIEFWKQVPPSEPYRVIIGDVRDKLYHTRERTRQLLSNGFSEIPEEATYTNIKQFLEPLELCYRSLCACGDRPIADGSLLDFLRQVSTFGLSLVRLDIRQESDRHTDVLDAITQHLEIGSYREWSEERKQEWLLSELSGKRPLFGPDLPKTEEIDDVLNTFHVISELPSDCFGAYIISMATAPSDVLAVELLQRECHVKQPLRVVPLFEKLDDLDAAPAAIARLFSIEWYKNRINGKQEVMIGYSDSGKDAGRLSAAWQLYKAQEELIKVAKQYDVKLIMFHGRGGTVGRGGGPSHLAILSQPPDTIQGSLRVTVQGEVIEQSFGEEHLCFRTLQRFTSATLEHGMYPPFPPKPEWRALLDEISVVATEKYRSIVFKEPRFVEYFRLATPELEYGRMNIGSRPSKRKRNGGIETLRAIPWIFAWTQTRFHLPVWLGFGAAFKYALNKDKKNLNMLRDMHKKWPFFRVTADLIEMVFAKGDPGIAALYDKLLVSEDLWSFGELLRSDYEETKSLLLKVAGHKELLENDPSLKQRLRLRDSYITTLNVCQAYTLKRIRDPTYKVTPRPHITKEYIESKPAAELVSLNPQSEYAPGLEDTLILTMKGIAAGMQNTG*