Gene Details:
Functional Descriptions:
- Overexpression of GCN2-type protein kinase (TaGCN2) in wheat has profound effects on free amino acid concentration and gene expression.
- GCN2-type PCR product (TaGCN2) was amplified from wheat (Triticum aestivum) RNA, while a wheat eIF2α homologue was identified in wheat genome data and found to contain a conserved target site for phosphorylation by GCN2.
- TaGCN2 overexpression in transgenic wheat resulted in significant decreases in total free amino acid concentration in the grain, with free asparagine concentration in particular being much lower than in controls.
- The study demonstrates that TaGCN2 plays an important role in the regulation of genes encoding enzymes of amino acid biosynthesis in wheat and is the first to implicate GCN2-type protein kinases so clearly in sulphur signalling in any organism.
- It shows that manipulation of TaGCN2 gene expression could be used to reduce free asparagine accumulation in wheat grain and the risk of acrylamide formation in wheat products.
Function-related keywords:
- kinase , grain , protein-kinase , grain-protein , protein
Literature:
- Overexpression of GCN2-type protein kinase in wheat has profound effects on free amino acid concentration and gene expression. DOI: 10.1111/j.1467-7652.2011.00665.x ; PMID: 22070093
Related News:
Gene Resources:
Orthologs:
Sequences:
cDNA Sequence
CDS Sequence
Protein Sequence