Gene Details:

Functional Descriptions:

  • Overexpression of the ZmDEF1 gene increases the resistance to weevil larvae in transgenic maize seeds.
  • The Zea mays defensin 1 (ZmDEF1) gene encodes the defensin 1 protein, which can inhibit alpha-amylase in the insect gut.
  • ZmDEF1 was cloned into a pBetaPhaso-dest vector, which harbours phaseolin, a seed-specific promoter, and the Agrobacterium tumefaciens strain C58 harbouring the pBetaPhaso-ZmDEF1 vector was used to transfer the ZmDEF1 gene into two maize cultivars using immature embryos.
  • The recombinant ZmDEF1 protein inhibited the alpha-amylase activity of the maize weevil larvae, and its ability to inhibit alpha-amylase is 54.52–63.09% greater than the ZmDEF1 protein extracted from non-transgenic plants.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >Zm00001eb247820_T002 GTATCCGCGAGGATCCCCCCACACCTTGCGACCTGCGTTGTCACCCACCCATCAAGGTTGGGGCCGCCAGCAGGTTCAGCCGTTCCTGTTCTTGATAAAACGAGAGAAGGATGGCGGCAGTGTCTCAGGGAGCTGTCCTATTCTTGTTTCTCCTCCTCGTCGCAGCAGCCATGCCCATGCATGCCTTGATAATGGAGAACGTGAAACAGCAGGAGAAGGAGAAGGAGAAGGAGAAGGAGGAGAAAAGCACGGAGAAGGAAGAGAGTCGATGCTTATCGCAGAGTCTCCAGTTCGAGGGCTTCTGCTTCAACAGCGACAGATGCGCCGAGGTGTGCATGAAGGAGAGCTTTCCCGGTGGCGAGTGCAAGCGGGACGTGGCCATGCGCAAGTGCTTCTGCAAGAAGCCTTGCTAGTTCATCGGTCTTGCTAATTGTTGATGGGTGCTTCATTAATTTGATTTGATGGGTCTCATGGCTACGAGAAACAAAACTACTTTTGATAAAATGCAGGGCATGAAAAGCGTTTCTGCAGTCCTTAAATTGCTGGGTTTTTTTTTGACGAAACTGCATGGAGGGGTTTGCAGCCCCTT
  • >Zm00001eb247820_T003 GTATCCGCGAGGATCCCCCCACACCTTGCGACCTGCGTTGTCACCCACCCATCAAGGTTGGGGCCGCCAGCAGGTTCAGCCGTTCCTGTTCTTGATAAAACGAGAGAAGGATGGCGGCAGTGTCTCAGGGAGCTGTCCTATTCTTGTTTCTCCTCCTCGTCGCAGCAGCCATGCCCATGCATGCCTTGATAATGGAGAACGTGAAACAGCAGGAGAAGGAGAAGGAGAAGGAGAAGGAGGAGAAAAGCACGGAGAAGGAAGAGAGTCGATGCTTATCGCAGAGTCTCCAGTTCGAGGGCTTCTGCTTCAACAGCGACAGATGCGCCGAGGTGTGCATGAAGGAGAGCTTTCCCGGTGGCGAGTGCAAGCGGGACGTGGCCATGCGCAAGTGCTTCTGCAAGAAGCCTTGCTAGTTCATCGGTCTTGCTAATTGTTGATGGGTGCTTCATTAATTTGATTTGATGGGTCTCATGGCTACGAGAAACAAAACTACTTTTGATAAAATGCAGGGCATGAAAAGCGTTTCTGCAGTCCTTAAATTGCTGGGTTTTTTTTTGACGAAACTGCATGGAGGGGTTTGCAGCCCCTT
  • >Zm00001eb247820_T001 GTATCCGCGAGGATCCCCCCACACCTTGCGACCTGCGTTGTCACCCACCCATCAAGGTTGGGGCCGCCAGCAGGTTCAGCCGTTCCTGTTCTTGATAAAACGAGAGAAGGATGGCGGCAGTGTCTCAGGGAGCTGTCCTATTCTTGTTTCTCCTCCTCGTCGCAGCAGCCATGCCCATGCATGCCTTGATAATGGAGAACGTGAAACAGCAGGAGAAGGAGAAGGAGAAGGAGAAGGAGGAGAAAAGCACGGAGAAGGAAGAGAGTCGATGCTTATCGCAGAGTCTCCAGTTCGAGGGCTTCTGCTTCAACAGCGACAGATGCGCCGAGGTGTGCATGAAGGAGAGCTTTCCCGGTGGCGAGTGCAAGCGGGACGTGGCCATGCGCAAGTGCTTCTGCAAGAAGCCTTGCTAGTTCATCGGTCTTGCTAATTGTTGATGGGTGCTTCATTAATTTGATTTGATGGGTCTCATGGCTACGAGAAACAAAACTACTTTTGATAAAATGCAGGGCATGAAAAGCGTTTCTGCAGTCCTTAAATTGCTGGGTTTTTTTTTGACGAAACTGCATGGAGGGGTTTGCAGCCCCTT
CDS Sequence
  • >Zm00001eb247820_T002 ATGGCGGCAGTGTCTCAGGGAGCTGTCCTATTCTTGTTTCTCCTCCTCGTCGCAGCAGCCATGCCCATGCATGCCTTGATAATGGAGAACGTGAAACAGCAGGAGAAGGAGAAGGAGAAGGAGAAGGAGGAGAAAAGCACGGAGAAGGAAGAGAGTCGATGCTTATCGCAGAGTCTCCAGTTCGAGGGCTTCTGCTTCAACAGCGACAGATGCGCCGAGGTGTGCATGAAGGAGAGCTTTCCCGGTGGCGAGTGCAAGCGGGACGTGGCCATGCGCAAGTGCTTCTGCAAGAAGCCTTGCTAG
  • >Zm00001eb247820_T003 ATGGCGGCAGTGTCTCAGGGAGCTGTCCTATTCTTGTTTCTCCTCCTCGTCGCAGCAGCCATGCCCATGCATGCCTTGATAATGGAGAACGTGAAACAGCAGGAGAAGGAGAAGGAGAAGGAGAAGGAGGAGAAAAGCACGGAGAAGGAAGAGAGTCGATGCTTATCGCAGAGTCTCCAGTTCGAGGGCTTCTGCTTCAACAGCGACAGATGCGCCGAGGTGTGCATGAAGGAGAGCTTTCCCGGTGGCGAGTGCAAGCGGGACGTGGCCATGCGCAAGTGCTTCTGCAAGAAGCCTTGCTAG
  • >Zm00001eb247820_T001 ATGGCGGCAGTGTCTCAGGGAGCTGTCCTATTCTTGTTTCTCCTCCTCGTCGCAGCAGCCATGCCCATGCATGCCTTGATAATGGAGAACGTGAAACAGCAGGAGAAGGAGAAGGAGAAGGAGAAGGAGGAGAAAAGCACGGAGAAGGAAGAGAGTCGATGCTTATCGCAGAGTCTCCAGTTCGAGGGCTTCTGCTTCAACAGCGACAGATGCGCCGAGGTGTGCATGAAGGAGAGCTTTCCCGGTGGCGAGTGCAAGCGGGACGTGGCCATGCGCAAGTGCTTCTGCAAGAAGCCTTGCTAG
Protein Sequence
  • >Zm00001eb247820_P002 MAAVSQGAVLFLFLLLVAAAMPMHALIMENVKQQEKEKEKEKEEKSTEKEESRCLSQSLQFEGFCFNSDRCAEVCMKESFPGGECKRDVAMRKCFCKKPC
  • >Zm00001eb247820_P003 MAAVSQGAVLFLFLLLVAAAMPMHALIMENVKQQEKEKEKEKEEKSTEKEESRCLSQSLQFEGFCFNSDRCAEVCMKESFPGGECKRDVAMRKCFCKKPC
  • >Zm00001eb247820_P001 MAAVSQGAVLFLFLLLVAAAMPMHALIMENVKQQEKEKEKEKEEKSTEKEESRCLSQSLQFEGFCFNSDRCAEVCMKESFPGGECKRDVAMRKCFCKKPC