Gene Details:

• Gene ID: Zm00001eb165710
• Gene Symbol: ZmNL4
• Gene Name: narrow leaf4
• Genome: Zm-B73-REFERENCE-NAM-5.0
• Species: Zea mays

Functional Descriptions:

• Map-based cloning revealed that ZmNL4, a kelch-repeat superfamily gene, emerged to be the candidate for qLW4a, and a single-base deletion in the conserved SMC_prok_B domain of ZmNL4 in Lin387 caused a frame shift, leading to premature termination.
• The knockout of ZmNL4 by CRISPR/Cas9 editing significantly reduced the LW, which was attributed to a reduction in the cell number instead of cell size, indicating a role of ZmNL4 in regulating cell division.
• ZmNL4 regulated leaf width and other important agronomic traits.
• Transcriptomic comparison of ZmNL4 knockout lines with the wild type B73-329 revealed that ZmNL4 might participate in cell wall biogenesis, asymmetric cell division, metabolic processes, transmembrane transport and response to external stimulus, etc.
• A kelch-repeat superfamily gene, ZmNL4, controls leaf width in maize.
• These results provide insights into the genetic and molecular mechanisms of ZmNL4 in controlling LW and could potentially contribute to optimizing plant architecture for maize breeding.

Function-related keywords:

• leaf ,  map-based-cloning ,  cell-division ,  architecture ,  breeding ,  cell-wall ,  plant-architecture ,  leaf-size

Literature:

• A kelch-repeat superfamily gene, ZmNL4, controls leaf width in maize (Zea mays L.).   DOI:  10.1111/tpj.15348 ;  PMID:  34009654
• Using High-Throughput Phenotyping Analysis to Decipher the Phenotypic Components and Genetic Architecture of Maize Seedling Salt Tolerance.   DOI:  10.3390/genes14091771 ;  PMID:  37761911

Related News:

• Plant Journal | 中国农业大学张义荣/邢界文发现调控玉米叶宽的重要数量性状位点

Gene Resources:

• NCBI ID: 100279529
• UniProt accessions: A0A1D6PPA7 ,  A0A1D6PP99 ,  A0A1D6PPA1

Sequences: