Gene Details:

• Gene ID: Zm00031ab118460
• Gene Symbol: ZmRF5
• Gene Name:
• Genome: Zm-B73-REFERENCE-NAM-5.0
• Species: Zea mays

Functional Descriptions:

• The maize CMS-C trait is regulated by the chimeric mitochondrial gene, atp6c, and can be recovered by the restorer gene ZmRF5.
• ZmRF5 cooperates with MORF8 and RS31A to restore CMS-C fertility in maize.
• The over-expression of ZmRF5 brought back the fertility to CMS-C plants, whereas its genomic editing by CRISPR/Cas9 induced abortive pollens in the restorer line.
• ZmRF5 is sorted to mitochondria, and recruited RS31A, a splicing factor, through MORF8 to form a cleaving/restoring complex, which promoted the cleaving of the CMS-associated transcripts atp6c by shifting the major cleavage site from 480th nt to 344 th nt for fast degradation, and preserved just right amount of atp6c RNA for protein translation, providing adequate ATP6C to assembly complex V, thus restoring male fertility.

Function-related keywords:

• fertility ,  mitochondria ,  male-fertility ,  protein-translation

Literature:

• A P-type pentatricopeptide repeat protein ZmRF5 promotes 5' region partial cleavages of atp6c transcripts to restore the fertility of CMS-C maize by recruiting a splicing factor.   DOI:  10.1111/pbi.14263 ;  PMID:  38073308

Related News:

• PBJ|汤继华团队揭示ZmRF5与MORF8和RS31A形成切割/育性恢复复合体对atp6c 5'区域部分切割并恢复育性的机制

Gene Resources:

• NCBI ID: 100502534
• UniProt accessions: A0A1D6F748

Sequences: